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dynamic array integrated fluidic circuit ifc chips  (fluidigm)


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    Structured Review

    fluidigm dynamic array integrated fluidic circuit ifc chips
    Dynamic Array Integrated Fluidic Circuit Ifc Chips, supplied by fluidigm, used in various techniques. Bioz Stars score: 94/100, based on 313 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/48+48+dynamic+array+chips/48%2E48+Dynamic+Array+IFC+for+Gene+Expression/pm41866099-141-7-22
    Average 94 stars, based on 313 article reviews
    dynamic array integrated fluidic circuit ifc chips - by Bioz Stars, 2026-09
    94/100 stars

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    Related Articles

    Polymerase Chain Reaction:

    Article Title: Reduction of systematic bias in transcriptome data from human peripheral blood mononuclear cells for transportation and biobanking.
    Article Snippet: The remaining five genes were selected because these genes have been suggested to be biomarkers of human disease (IL6, SLC6A4, HDAC5, NR3C1, and BDNF). cDNA was synthesized from RNA samples using a SuperScript VILO cDNA Synthesis Kit (Life Technologies). .. For a combination of 48 samples648 pairs of PCR primers, pre-amplification of cDNA and qRT-PCR experiments were performed in parallel BioMark 48.48 Dynamic Array chips (Fluidigm, South San Francisco, CA, USA). ..

    Quantitative RT-PCR:

    Article Title: Reduction of systematic bias in transcriptome data from human peripheral blood mononuclear cells for transportation and biobanking.
    Article Snippet: The remaining five genes were selected because these genes have been suggested to be biomarkers of human disease (IL6, SLC6A4, HDAC5, NR3C1, and BDNF). cDNA was synthesized from RNA samples using a SuperScript VILO cDNA Synthesis Kit (Life Technologies). .. For a combination of 48 samples648 pairs of PCR primers, pre-amplification of cDNA and qRT-PCR experiments were performed in parallel BioMark 48.48 Dynamic Array chips (Fluidigm, South San Francisco, CA, USA). ..

    Gene Expression:

    Article Title: Follicular dendritic cell activation by TLR ligands promotes autoreactive B cell responses
    Article Snippet: Data were analyzed with Volocity software (Perkin-Elmer) or ImageJ. .. Gene expression analysis was performed using TaqMan Gene Expression Assays in the BioMark 48.48 Dynamic Array chips (Fluidigm Corp.) Delta-delta Cts (ΔΔCt) were calculated using the mean of two reference genes (18S and GAPDH) and converted to fold change by the 2−ΔΔCt formula relative to the B6 control. qPCR (RT-PCR) analyses of Ifna, Il6, and Ifnb1 were performed using the SYBR-green system. ..

    Article Title: Follicular dendritic cell activation by TLR ligands promotes autoreactive B cell responses
    Article Snippet: Data were analyzed with Volocity software (Perkin-Elmer) or ImageJ. .. Gene expression analysis Gene expression analysis was performed using TaqMan Gene Expression Assays in the BioMark 48.48 Dynamic Array chips (Fluidigm Corp.) Delta-delta Cts (ΔΔCt) were calculated using the mean of two reference genes (18S and GAPDH) and converted to fold change by the 2−ΔΔCt formula relative to the B6 control. qPCR (RT-PCR) analyses of Ifna, Il6, and Ifnb1 were performed using the SYBR-green system. ..

    Article Title: Type I IFNs Regulate Inflammation, Vasculopathy, and Fibrosis in Chronic Cutaneous Graft-versus-Host Disease.
    Article Snippet: Preamplification of cDNAs and real-time PCR assays were done as follows: a mixture of 14 TaqMan gene expression assays (Applied Biosystems) was prepared using the TaqMan PreAmp Master Mix kit (Applied Biosystems). .. A total of 37 samples were run in triplicate using a set of 14 TaqMan gene expression assays in the BioMark 48.48 Dynamic Array chips (Fluidigm, San Francisco, CA). .. Dynamic arrays were loaded using a NanoFlex 4-IFC controller (Fluidigm), and real-time reactions were performed and analyzed using the BioMark real-time PCR system and analysis software (Fluidigm), respectively.

    Control:

    Article Title: Follicular dendritic cell activation by TLR ligands promotes autoreactive B cell responses
    Article Snippet: Data were analyzed with Volocity software (Perkin-Elmer) or ImageJ. .. Gene expression analysis was performed using TaqMan Gene Expression Assays in the BioMark 48.48 Dynamic Array chips (Fluidigm Corp.) Delta-delta Cts (ΔΔCt) were calculated using the mean of two reference genes (18S and GAPDH) and converted to fold change by the 2−ΔΔCt formula relative to the B6 control. qPCR (RT-PCR) analyses of Ifna, Il6, and Ifnb1 were performed using the SYBR-green system. ..

    Article Title: Follicular dendritic cell activation by TLR ligands promotes autoreactive B cell responses
    Article Snippet: Data were analyzed with Volocity software (Perkin-Elmer) or ImageJ. .. Gene expression analysis Gene expression analysis was performed using TaqMan Gene Expression Assays in the BioMark 48.48 Dynamic Array chips (Fluidigm Corp.) Delta-delta Cts (ΔΔCt) were calculated using the mean of two reference genes (18S and GAPDH) and converted to fold change by the 2−ΔΔCt formula relative to the B6 control. qPCR (RT-PCR) analyses of Ifna, Il6, and Ifnb1 were performed using the SYBR-green system. ..

    Real-time Polymerase Chain Reaction:

    Article Title: Follicular dendritic cell activation by TLR ligands promotes autoreactive B cell responses
    Article Snippet: Data were analyzed with Volocity software (Perkin-Elmer) or ImageJ. .. Gene expression analysis was performed using TaqMan Gene Expression Assays in the BioMark 48.48 Dynamic Array chips (Fluidigm Corp.) Delta-delta Cts (ΔΔCt) were calculated using the mean of two reference genes (18S and GAPDH) and converted to fold change by the 2−ΔΔCt formula relative to the B6 control. qPCR (RT-PCR) analyses of Ifna, Il6, and Ifnb1 were performed using the SYBR-green system. ..

    Article Title: Effects of cellular origin on differentiation of human induced pluripotent stem cell–derived endothelial cells
    Article Snippet: The program was run in an Applied Biosystems Veriti thermal cycler as follows: 50°C for 15 minutes, 70°C for 2 minutes, and 94°C for 2 minutes; 94°C for 15 seconds, 60°C for 30 seconds, and 68°C for 45 seconds for 18 cycles; followed by 68°C for 7 minutes. .. The preamplified cDNA was loaded onto Biomark 48.48 Dynamic Array chips (Fluidigm) using the Nanoflex IFC controller (Fluidigm). qPCR was performed on a BioMark HD System (Fluidigm), and data were processed by BioMark Real-Time qPCR Analysis software. ..

    Article Title: Cardiomyocytes from induced pluripotent stem cells from patients and methods of use thereof
    Article Snippet: .. Amplified cDNA was loaded into Biomark 48.48 Dynamic Array chips (Fluidigm, South San Francisco, Calif.) for analysis by the BioMark Real-Time PCR Analysis software (Fluidigm). .. Calcium (Ca2+) imaging. iPSC-CMs were dissociated and seeded in gelatin-coated 8-well LAB-TEK® II chambers (Nalgene Nunc International) for calcium imaging.

    Article Title: Heterogeneously Expressed fezf2 Patterns Gradient Notch Activity in Balancing the Quiescence, Proliferation, and Differentiation of Adult Neural Stem Cells
    Article Snippet: .. FACS sorted cells were preselected by semiquantitative RT-PCR for fezf2 expression and then subjected to nanofluidic real-time PCR using the Fluidigm 48.48 Dynamic Array Chips following the manufacturer's instructions ( Spurgeon et al., 2008 ). ..

    Article Title: Follicular dendritic cell activation by TLR ligands promotes autoreactive B cell responses
    Article Snippet: Data were analyzed with Volocity software (Perkin-Elmer) or ImageJ. .. Gene expression analysis Gene expression analysis was performed using TaqMan Gene Expression Assays in the BioMark 48.48 Dynamic Array chips (Fluidigm Corp.) Delta-delta Cts (ΔΔCt) were calculated using the mean of two reference genes (18S and GAPDH) and converted to fold change by the 2−ΔΔCt formula relative to the B6 control. qPCR (RT-PCR) analyses of Ifna, Il6, and Ifnb1 were performed using the SYBR-green system. ..

    Software:

    Article Title: Effects of cellular origin on differentiation of human induced pluripotent stem cell–derived endothelial cells
    Article Snippet: The program was run in an Applied Biosystems Veriti thermal cycler as follows: 50°C for 15 minutes, 70°C for 2 minutes, and 94°C for 2 minutes; 94°C for 15 seconds, 60°C for 30 seconds, and 68°C for 45 seconds for 18 cycles; followed by 68°C for 7 minutes. .. The preamplified cDNA was loaded onto Biomark 48.48 Dynamic Array chips (Fluidigm) using the Nanoflex IFC controller (Fluidigm). qPCR was performed on a BioMark HD System (Fluidigm), and data were processed by BioMark Real-Time qPCR Analysis software. ..

    Article Title: Cardiomyocytes from induced pluripotent stem cells from patients and methods of use thereof
    Article Snippet: .. Amplified cDNA was loaded into Biomark 48.48 Dynamic Array chips (Fluidigm, South San Francisco, Calif.) for analysis by the BioMark Real-Time PCR Analysis software (Fluidigm). .. Calcium (Ca2+) imaging. iPSC-CMs were dissociated and seeded in gelatin-coated 8-well LAB-TEK® II chambers (Nalgene Nunc International) for calcium imaging.

    Amplification:

    Article Title: Cardiomyocytes from induced pluripotent stem cells from patients and methods of use thereof
    Article Snippet: .. Amplified cDNA was loaded into Biomark 48.48 Dynamic Array chips (Fluidigm, South San Francisco, Calif.) for analysis by the BioMark Real-Time PCR Analysis software (Fluidigm). .. Calcium (Ca2+) imaging. iPSC-CMs were dissociated and seeded in gelatin-coated 8-well LAB-TEK® II chambers (Nalgene Nunc International) for calcium imaging.

    FACS:

    Article Title: Heterogeneously Expressed fezf2 Patterns Gradient Notch Activity in Balancing the Quiescence, Proliferation, and Differentiation of Adult Neural Stem Cells
    Article Snippet: .. FACS sorted cells were preselected by semiquantitative RT-PCR for fezf2 expression and then subjected to nanofluidic real-time PCR using the Fluidigm 48.48 Dynamic Array Chips following the manufacturer's instructions ( Spurgeon et al., 2008 ). ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Heterogeneously Expressed fezf2 Patterns Gradient Notch Activity in Balancing the Quiescence, Proliferation, and Differentiation of Adult Neural Stem Cells
    Article Snippet: .. FACS sorted cells were preselected by semiquantitative RT-PCR for fezf2 expression and then subjected to nanofluidic real-time PCR using the Fluidigm 48.48 Dynamic Array Chips following the manufacturer's instructions ( Spurgeon et al., 2008 ). ..

    Expressing:

    Article Title: Heterogeneously Expressed fezf2 Patterns Gradient Notch Activity in Balancing the Quiescence, Proliferation, and Differentiation of Adult Neural Stem Cells
    Article Snippet: .. FACS sorted cells were preselected by semiquantitative RT-PCR for fezf2 expression and then subjected to nanofluidic real-time PCR using the Fluidigm 48.48 Dynamic Array Chips following the manufacturer's instructions ( Spurgeon et al., 2008 ). ..



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